Centaurin Alpha 2 (CENTa2) is a member of the Centaurin family of proteins, which are characterized by their unique structure and function in intracellular signaling and endosomal trafficking. It plays a role in various cellular processes by interacting with other molecules and is implicated in the regulation of cell growth, migration, and differentiation.
Besides,Neurofibromin 1 (NF1) has been identified as an interactor of CENTa2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CENTa2 and recombinant human NF1. Briefly, CENTa2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NF1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CENTa2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse CENTa2 and recombinant human NF1 was shown in Figure 1, the EC50 for this effect is 2.96ug/mL.