中间α-球蛋白抑制因子H4(ITIH4)活性蛋白
Active Inter Alpha-Globulin Inhibitor H4 (ITIH4)
IHRP; PK120; H4P; ITIHL1; Plasma Kallikrein-Sensitive Glycoprotein; Plasma kallikrein sensitive glycoprotein 120; Inter-alpha-trypsin inhibitor family heavy chain-related protein
- 编号APH776Hu03
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点6.9
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1728 ¥ 4320 ¥ 8640 ¥ 25920 ¥ 64800
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
Figure. Western Blot
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Figure. The binding activity of ITIH4 with Grb2.
Inter Alpha-Globulin Inhibitor H4 (ITIH4) is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. ITIH4 has been detected only in liver, and it seems to be upregulated during surgical trauma. It may also play a role in liver development or regeneration. Besides, Growth Factor Receptor Bound Protein 2 (Grb2) has been identified as an interactor of ITIH4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ITIH4 and recombinant human Grb2. Briefly, ITIH4 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to
Grb2-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ITIH4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ITIH4 and Grb2 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APH776Hu01 | 中间α-球蛋白抑制因子H4(ITIH4)活性蛋白 | Cell culture; Activity Assays. |
| APH776Hu03 | 中间α-球蛋白抑制因子H4(ITIH4)活性蛋白 | Cell culture; Activity Assays. |
| RPH776Hu02 | 中间α-球蛋白抑制因子H4(ITIH4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH776Hu03 | 中间α-球蛋白抑制因子H4(ITIH4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPH776Hu01 | 中间α-球蛋白抑制因子H4(ITIH4)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAH776Hu03 | 中间α-球蛋白抑制因子H4(ITIH4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH776Hu02 | 中间α-球蛋白抑制因子H4(ITIH4)多克隆抗体 | WB; IHC; ICC; IP. |
| PAH776Hu01 | 中间α-球蛋白抑制因子H4(ITIH4)多克隆抗体 | WB; IHC; ICC; IP. |
| LAH776Hu71 | 中间α-球蛋白抑制因子H4(ITIH4)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAH776Hu21 | 中间α-球蛋白抑制因子H4(ITIH4)单克隆抗体 | WB; IHC; ICC; IP. |
| SEH776Hu | 中间α-球蛋白抑制因子H4(ITIH4)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMH776Hu | 中间α-球蛋白抑制因子H4(ITIH4)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSH776Hu01 | 中间α-球蛋白抑制因子H4(ITIH4)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Toxicology Letters | Biomarkers for methotrexate-induced liver injury: urinary protein profiling of psoriasis patients. [Pubmed: 23830989] |
| International Journal of Medical Microbiology | Proteomic demonstration of the recurrent presence of inter-alpha-inhibitor H4 heavy-chain during aspergillosis induced in an animal model [Pubmed:24360996] |
| Cancer Research and Treatment | Inter-alpha Inhibitor H4 as a Potential Biomarker Predicting the Treatment Outcomes in Patients with Hepatocellular Carcinoma [pubmed:28724284] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
| Liver Cancer | Biomarkers for locally advanced hepatocellular carcinoma patients treated with liver-directed combined radiotherapy [] |