白介素17受体C(IL17RC)活性蛋白
Active Interleukin 17 Receptor C (IL17RC)
IL17-RL; IL17R-C; IL17Rhom; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; ZcytoR14
- 编号APD350Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 97%
- 等电点5.8
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1920 ¥ 4800 ¥ 9600 ¥ 28800 ¥ 72000
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产品包装(模拟)
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产品包装(模拟)
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Figure. SDS-PAGE; Sample: Active recombinant IL17RC, Human.
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Figure. Western Blot; Sample: Recombinant IL17RC, Human.
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通过ISO 9001、ISO 13485质量体系认证
活性实验
IL17RC (Interleukin-17 receptor C) is a receptor for IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RA. This protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. A binding ELISA assay was conducted to detect the association of IL17RC with IL17RA. Briefly, IL17RC were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RC with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPD350Hu01 | 白介素17受体C(IL17RC)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD350Hu01 | 白介素17受体C(IL17RC)活性蛋白 | Cell culture; Activity Assays. |
| PAD350Hu01 | 白介素17受体C(IL17RC)多克隆抗体 | WB |
参考文献
| 杂志 | 参考文献 |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … [Pubmed:29375374] |