表皮生长因子受体2(ErbB2)活性蛋白
Active Receptor Tyrosine Protein Kinase erbB-2 (ErbB2)
CD340; EGFR2; HER-2/neu; HER2; NEU; MLN 19; NGL; TKR1; p185; Epidermal Growth Factor Receptor 2; V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2; Neuro/Glioblastoma Derived Oncogene Homolog
- 编号APB867Hu02
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性状冻干粉
- 纯度> 90%
- 等电点6.4
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
Figure. SDS-PAGE
-
通过ISO 9001、ISO 13485质量体系认证
活性实验
Receptor Tyrosine Protein Kinase erbB-2 (ErbB2) as known as HER2 (Human Epidermal Growth Factor Receptor 2), is a member of the EGFR family of receptor tyrosine kinases. It is a 185 kDa cell membrane receptor that plays a crucial role in cell signaling and proliferation.Besides,Plakophilin 2 (PKP2) has been identified as an interactor of ErbB2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ErbB2 and recombinant human PKP2. Briefly, ErbB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PKP2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ErbB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human ErbB2 and recombinant human PKP2 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB867Hu01 | 表皮生长因子受体2(ErbB2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB867Hu02 | 表皮生长因子受体2(ErbB2)活性蛋白 | Cell culture; Activity Assays. |
| APB867Hu01 | 表皮生长因子受体2(ErbB2)活性蛋白 | Cell culture; Activity Assays. |
| RPB867Hu02 | 表皮生长因子受体2(ErbB2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB867Hu02 | 表皮生长因子受体2(ErbB2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB867Hu01 | 表皮生长因子受体2(ErbB2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB867Hu81 | 表皮生长因子受体2(ErbB2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB867Hu71 | 表皮生长因子受体2(ErbB2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB867Hu24 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB867Hu27 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB867Hu29 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB867Hu23 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB867Hu21 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB867Hu22 | 表皮生长因子受体2(ErbB2)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB867Hu | 表皮生长因子受体2(ErbB2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB867Hu | 表皮生长因子受体2(ErbB2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Neuroscience Letter | Therapeutic effect of co-transplantation of neuregulin 1-transfected-Schwann cells and bone marrow stromal cells on spinal cord hemisection syndrom [Cyagen: source] |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample [] |